𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The effect of novobiocin on yeast topoisomerase type II

✍ Scribed by Pocklington, Michael J. ;Jenkins, John R. ;Orr, Elisha

Publisher
Springer
Year
1990
Tongue
English
Weight
1016 KB
Volume
220
Category
Article
ISSN
0026-8925

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✦ Synopsis

Low concentrations of novobiocin are toxic to permeable yeast cells, but do not inhibit type II topoisomerase activity. Furthermore, the enzyme does not bind specifically to novobiocin-Sepharose. These observations are in agreement with genetical analyses. Mutations at a single locus that confer novobiocin resistance and temperature sensitivity exhibit a similar phenotype to cells treated with novobiocin, but are not topoisomerase II mutants.

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