✦ LIBER ✦

Effects of topoisomerase I inhibition on the expression of topoisomerase IIα measured with fluorescence image cytometry

✍ Scribed by Trudey Nicklee; Michael Crump; David W. Hedley

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 543 KB
Volume: 25
Category: Article
ISSN: 0196-4763
DOI: 10.1002/(sici)1097-0320(19961101)25:3<205::aid-cyto1>3.0.co;2-e

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✦ Synopsis

A fluorescence image cytometry technique was developed to measure the effects of topotecan, a topoisomerase I inhibitor, on the nuclear expression of topoisomerase IIa in a series of patients with refractory or relapsed acute myeloid leukemia (AML). W e used a commercially available affinitypuritied rabbit polyclonal antibody and a fluorescein-conjugated secondary antibody. By using DAPI as a DNA counterstain and dual wavelength excitation, it was possible to measure enzyme expression

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