## SYNOPSIS DNA molecules ranging in size from 1 to 630 kilobase pair and iptercalated with either ethidium bromide (EtBr) or propidium iSdide (PI) were electrophoresed in 1% agarose at four different electric field strengths. The extent of intercalation of EtBr under the conditions of our electro
The effect of charges on the melting of DNA
โ Scribed by Mitsuru Nagasawa; Yoshio Muroga
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1972
- Tongue
- English
- Weight
- 735 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0006-3525
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โฆ Synopsis
A method of calculating both the electrostatic free energy and enthalpy changes for melting of DNA is proposed. The nonelectrostatic parts of free energy and enthalpy changes for melting of DNA are obtained by huhtracting these electrostatic free-energy and enthalpy changes from the total free-energy and enthalpy changes of melting reported by Privalov, Ptitsyn, and Birsht ein, respectively. The ionic strength dependence of the melting temperature of DNA is computed using these thermodynamic functions.
๐ SIMILAR VOLUMES
## Synopsis Systematic experimental data for the dependence of melting range width AT for phage T2 and calf thymus DNA's on sodium ion concentration was obtained. A mathematical description of helix-coil transition was given for the case of DNA with a block sequence of nucleotides (such as calf th