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Effect of ionic conditions on DNA melting curve parameters

✍ Scribed by I. V. Berestetskaya; M. D. Frank-Kamenetskii; Yu. S. Lazurkin

Publisher
Wiley (John Wiley & Sons)
Year
1974
Tongue
English
Weight
558 KB
Volume
13
Category
Article
ISSN
0006-3525

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✦ Synopsis

Synopsis

Systematic experimental data for the dependence of melting range width AT for phage T2 and calf thymus DNA's on sodium ion concentration was obtained. A mathematical description of helix-coil transition was given for the case of DNA with a block sequence of nucleotides (such as calf thymus DNA). This description predicts an almost linear dependence of A T for DNA from higher organisms on the value TGC-TAT. On the other hand, AT is proportional to (TccTAT)'.~ for DNA's with quasi-random sequences (such as phage T2 DNA). Experimental data fully confirmed the theoretical predictions.

The most striking feature of this dependence is the sharp rise in TGC-TAT in the region of low sodium ion concentration (<3 X

The dependence of TGC and of TAT on ionic strength was calculated separately. In the region of low ionic strength, these quantities show markedly different behavior.

The dependence of TGC-TAT on sodium ion concentration was determined.

up to 100Β°C.

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