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Effects of DNA charge and length on the electrophoretic mobility of intercalated DNA

✍ Scribed by Howard R. Reese

Publisher
Wiley (John Wiley & Sons)
Year
1994
Tongue
English
Weight
914 KB
Volume
34
Category
Article
ISSN
0006-3525

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✦ Synopsis

SYNOPSIS

DNA molecules ranging in size from 1 to 630 kilobase pair and iptercalated with either ethidium bromide (EtBr) or propidium iSdide (PI) were electrophoresed in 1% agarose at four different electric field strengths. The extent of intercalation of EtBr under the conditions of our electrophoresis experiments was determined by a spectroscopic technique, whereas the extent of intercalation of PI was inferred from previous studies. The effects of the increase in DNA contour length and the concomitant decrease of linear charge density were separated based on our analysis of the mobility data. We conclude that the main factor responsible for the reduced electrophoretic mobility of intercalated DNA is the diminished linear charge density and not the increased contour length.

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