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The DnaA protein determines the initiation mass of Escherichia coli K-12

✍ Scribed by Løbner-Olesen, Anders; Skarstad, Kirsten; Hansen, Flemming G.; von Meyenburg, Kaspar; Boye, Erik

Book ID
122459722
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
913 KB
Volume
57
Category
Article
ISSN
0092-8674

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Initiation of DNA replication in Escherichia coli after overproduction of the DnaA protein
✍ Skarstad, Kirsten ;Løbner-Olesen, Anders ;Atlung, Tove ;von Meyenburg, Kaspar ;B 📂 Article 📅 1989 🏛 Springer 🌐 English ⚖ 718 KB

Flow cytometry was used to study initiation of DNA replication in Escherichia coli K12 after induced expression of a plasmid-borne dnaA+ gene. When the dnaA gene was induced from either the plac or the lambda pL promoter initiation was stimulated, as evidenced by an increase in the number of origins