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Membrane Attachment Activates dnaA Protein, the Initiation Protein of Chromosome Replication in Escherichia coli

✍ Scribed by Benjamin Yat-Ming Yung and Arthur Kornberg


Book ID
123635827
Publisher
National Academy of Sciences
Year
1988
Tongue
English
Weight
700 KB
Volume
85
Category
Article
ISSN
0027-8424

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Flow cytometry was used to study initiation of DNA replication in Escherichia coli K12 after induced expression of a plasmid-borne dnaA+ gene. When the dnaA gene was induced from either the plac or the lambda pL promoter initiation was stimulated, as evidenced by an increase in the number of origins