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ATP activates dnaA protein in initiating replication of plasmids bearing the origin of the E. coli chromosome

✍ Scribed by Sekimizu, Kazuhisa; Bramhill, David; Kornberg, Arthur


Book ID
123009021
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
698 KB
Volume
50
Category
Article
ISSN
0092-8674

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Initiation of DNA replication in Escheri
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Flow cytometry was used to study initiation of DNA replication in Escherichia coli K12 after induced expression of a plasmid-borne dnaA+ gene. When the dnaA gene was induced from either the plac or the lambda pL promoter initiation was stimulated, as evidenced by an increase in the number of origins