New Non-detrimental DNA-binding Mutants of the Escherichia coli Initiator Protein DnaA
β Scribed by M. Asklund; T. Atlung
- Book ID
- 116661807
- Publisher
- Elsevier Science
- Year
- 2005
- Tongue
- English
- Weight
- 744 KB
- Volume
- 345
- Category
- Article
- ISSN
- 0022-2836
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Flow cytometry was used to study initiation of DNA replication in Escherichia coli K12 after induced expression of a plasmid-borne dnaA+ gene. When the dnaA gene was induced from either the plac or the lambda pL promoter initiation was stimulated, as evidenced by an increase in the number of origins
A series of temperature-resistant revertants were isolated from strains of Escherichia coli K12 carrying a temperature-sensitive mutation in the dnaA gene. Four independent revertants were found which still carry the original ts mutation. The ability of these strains to grow at high temperature is d