The different roles of serum and calcium in the control of proliferation of BALB/C 3T3 mouse cells
β Scribed by A. L. Boynton; J. F. Whitfield; R. J. Isaacs
- Book ID
- 112895813
- Publisher
- Society for In Vitro Biology
- Year
- 1976
- Tongue
- English
- Weight
- 296 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1475-2689
No coin nor oath required. For personal study only.
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## Abstract Benzo[a]pyreneβtransformed Balb 3T3 cells (BP3T3) exhibit βnormalβ growth controls at low concentrations of serum. Epidermal growth factor (EGF) stimulates DNA synthesis and cell division in both Balb 3T3 and BP3T3 cells at physiological concentrations. The growth response of BP3T3 cell
BalbIc3T3 cells in crowded cultures detach from the dish when deprived of serum, and the survivors incorporate 3H-thymidine a t a reduced rate. The detachment becomes pronounced two hours after removal of serum, and reaches its maximum rate between two and four hours. Cells in sparse cul-