## Abstract The growth requirements of SV40 transformed Balb/cโ3T3 cells have been studied in the absence of serum. For growth in serumโfree medium, the cells require (i) insulin, (ii) transferrin, and (iii) cisโunsaturated fatty acids added in combination with fatty acid free bovine serum albumin.
Stimulatory effect of serum albumin on the proliferation of serum-free SV40-transformed Balb/c 3T3 cells
โ Scribed by Roberta Bedotti; Angelo F. Borghetti; Roberto Favilla
- Book ID
- 119121061
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 510 KB
- Volume
- 1053
- Category
- Article
- ISSN
- 0167-4889
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๐ SIMILAR VOLUMES
## Abstract The effect of serum on the growth properties of nonโtransformed Balb 3T3 A31 and SV40โtransformed Balb 3T3 A31 was studied. The concentration of serum in the growth medium of nonโtransformed cells had little effect on the initial population doubling time, but did regulate the cell densi
One of the earliest responses of quiescent mammalian cells to the addition of serum is an increase in intracellular p H (pH'"). This pH'" change is generally believed to be due to an increased activity of Na'/Hf exchange. A number of investigators have observed steady-state differences in pH'" betwe