The Development of a Data System for a Combination of Liquid Chromatography or Capillary Electrophoresis with an Ion Trap Storage/Reflectron Time-of-flight Mass Detector

✍ Scribed by Mark G. Qian; Jing-Tao Wu; Steve Parus; David M. Lubman

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 720 KB
• Volume: 10
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/(sici)1097-0231(19960731)10:10<1209::aid-rcm645>3.0.co;2-f

✦ Synopsis

A data system based upon a 200MHz transient recorder interface card in a Pentium PC computer is demonstrated for on-line analysis of microbore high-performance liquid chromatography (HPLC), capillary HPLC and capillary electrophoresis (CE) separations using a fast and sensitive ion-trap storage/reflectron timeof-flight mass spectrometric detector (IT-reTOFMS). Under the control of a user-written program, the system is capable of conducting the data acquisition and storage for a minimum of 30 min, at rates exceeding 10 Hz, of individual mass spectra containing 16000 data points having lOnsec resolution. The capability is mainly attributed to the use of a data reduction scheme in which only mass intensities higher than a preset threshold are saved as indexed flight-timelintensity pairs. This produces a typical reduction ratio of 309 in data set size, yielding faster storage with smaller file size, and permits the complete set of mass spectra to be held in the computer's memory. In addition, the data system is capable of displaying, for real-time evaluation of the analysis, each individual mass spectrum and the total-ion chromatogram. Further, the selected-ion chromatograms of given masses and a 3-dimensional topographic map describing a separation process can be rapidly generated from the collected data for the unambiguous and high fidelity identification of target analytes in a complex mixture.