✦ LIBER ✦

Development of a capillary high-performance liquid chromatography tandem mass spectrometry system using SWIFT technology in an ion trap/reflectron time-of-flight mass spectrometer

✍ Scribed by Ling He; Jing-Tao Wu; Steve Parus; David M. Lubman

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 207 KB
Volume: 11
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19971030)11:16<1739::aid-rcm77>3.0.co;2-3

No coin nor oath required. For personal study only.

✦ Synopsis

In this work a capillary high-performance liquid chromatography/tandem mass spectrometry system has been developed in a quadrupole ion trap/reflectron time-of-flight mass spectrometer. The broadband excitation for isolating a precursor ion is achieved by generating the stored waveform inverse Fourier transform waveform in real time. Following the isolation and cooling periods, an auxiliary AC voltage (tickle) is applied to the entrance end cap of the ion trap to generate collision induced dissociation. The amplitude of the tickle voltage is optimized on-line using a feedback method which involves having two computers communicating with each other. This system has been tested with a 4-peptide mixture and tandem mass spectra were obtained for each peptide.

📜 SIMILAR VOLUMES

Analysis of a commercial preparation of

Analysis of a commercial preparation of erythromycin estolates by tandem mass spectrometry and high performance liquid chromatography/electrospray ionization tandem mass spectrometry using an ion trap mass spectrometer

✍ Chien-Chen Lai; Pei-Lun Tsai; Cheng Yu; Guor-Rong Her 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 114 KB 👁 1 views

Because of the lack of a UV chromophore and their much smaller abundances in comparison with the major component, the minor components in erythromycin estolate preparations are difficult to analyze by high performance liquid chromatography ultraviolet (HPLC-UV). Tentative assignment of the major and

Identification of proteins from two-dime

Identification of proteins from two-dimensional gel electrophoresis of human erythroleukemia cells using capillary high performance liquid chromatography/electrospray-ion trap-reflectron time-of-flight mass spectrometry with two-dimensional topographic map analysis of in-gel tryptic digest products

✍ Yajuan Chen; Xiaoying Jin; David Misek; Robert Hinderer; Sam M. Hanash; David M. 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 268 KB 👁 1 views

Protein spots from two-dimensional (2-D) gel electrophoresis of a human erythroleukemia cell line have been identified by analysis of the in-gel tryptic digests using capillary high performance liquid chromatography (HPLC) separation with on-line detection using electrospray ionization mass spectrom