The Use of On-line Capillary Electrophoresis/Electrospray Ionization with Detection via an Ion Trap Storage/Reflectron Time-of-flight Mass Spectrometer for Rapid Mutation-site Analysis of Hemoglobin Variants
✍ Scribed by Michael X. Li; Jing-Tao Wu; Lin Liu; David M. Lubman
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 204 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0951-4198
No coin nor oath required. For personal study only.
✦ Synopsis
Capillary electrophoresis/electrospray ionization using an ion trap storage/reflectron time-of-flight mass spectrometer detector (CE/ESI-IT/reTOF) is used to provide a rapid and sensitive method for analyzing structural variants in the hemoglobin (Hb) β-chain. The Hb α-and β-chains are separated and the β-chain is digested by trypsin. The digest is analyzed by CE/ESI-IT/reTOF where a comparison of the total ion electrophorograms and mass spectra of the mutant and normal hemoglobins (Hbs) can detect the presence of a mutation site. In addition, collision-induced dissociation in the vacuum interface -skimmer region can be used to pinpoint the identity of such a site. The unique capability of the CE/ESI-IT/reTOF system for accurately detecting fast separations with narrow peaks that may be under 1 s full width at half maximum is demonstrated. The speed of this system is essential for resolution of the large number of peaks that are separated in a short time duration using CE separations.