THE DETERMINATION OF PICOMOLE AMOUNTS OF ACETYLCHOLINE IN MAMMALIAN BRAIN
โ Scribed by A. M. Goldberg; R. E. McCaman
- Book ID
- 111167367
- Publisher
- John Wiley and Sons
- Year
- 1973
- Tongue
- English
- Weight
- 475 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0022-3042
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
An enzymatic method for the determination of picomol amounts of dCMP in cellfree extracts was developed. The method is based on the quantitative phosphorylation of dCMP to dCTP by a partially purified dCMP-kinase and a pure NDP-kinase. The dCTP formed is measured using a DNApolymerase assay. With t
An assay capable of detecting tens-of-picomole quantities of choline and acetylcholine in milliliter volumes of a physiological salt solution has been developed. Silica column chromatography was used to bind and separate IO-3000 pmol ['4C]choline and [?Z]acetylcholine standards made up in 3 ml of a