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An Enzymatic Method for the Determination of dCTP and dGTP in Picomole Amounts

โœ Scribed by Lambert Skoog


Book ID
115112509
Publisher
John Wiley and Sons
Year
1970
Tongue
English
Weight
611 KB
Volume
17
Category
Article
ISSN
1432-1327

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An enzymatic method for the determinatio
โœ Kerstin Brynolf ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 578 KB

An enzymatic method for the determination of picomol amounts of dCMP in cellfree extracts was developed. The method is based on the quantitative phosphorylation of dCMP to dCTP by a partially purified dCMP-kinase and a pure NDP-kinase. The dCTP formed is measured using a DNApolymerase assay. With t