Although phospholipase C (PLC) is known to be activated by water-insoluble organic solvents, most activity assays have been designed to work in an aqueous milieu. Here a sensitive method is described for the determination of PLC activity in two-phase systems. The assay is based on the hydrolysis of
The Determination of Phospholipase D Activity in Emulsion Systems
β Scribed by Ines Aurich; Frank Hirche; Renate Ulbrich-Hofmann
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 69 KB
- Volume
- 268
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
Phospholipase D (PLD) is an enzyme which participates in the signaling mechanism cleaving phosphatidylcholine (PC) to choline and phosphatidic acid (PA). In Tetrahymena pyriformis GL this enzyme activity is enhanced by different kinds of agonists (sodium orthovanadate, sodium fluoride and phorbol 12
A nonradioactive assay for the investigation of phospholipase D (PLD) activity in cardiac membranes has been developed. A fluorescent derivative of phosphatidylcholine [2-decanoyl-1-(O-(11-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3proprionyl)amino) undecyl) sn-glycero-3-phosphocholin