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The determination of glucose-6-phosphatase activity in blood serum

✍ Scribed by J. Kellen; K. Belaj


Book ID
115816398
Publisher
Elsevier Science
Year
1961
Tongue
English
Weight
160 KB
Volume
6
Category
Article
ISSN
0009-8981

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We present a method to determine glucose 6-phosphate activity. This assay measures the rate of glucose released in the glucose-6-phosphatase reaction. The glucose is oxidized to beta-D-gluconolactone by glucose dehydrogenase in a coupled reaction that uses NAD(P)+. The determination is rapid, reprod

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A sensitive and rapid spectrophotometric method for determination of glucose-6-phosphatase activity is described. Glucose formed by the enzyme is oxidized by glucose oxidase to gluconolactone and hydrogen peroxide. The latter, phenol, and 4-aminoantipyrine are converted by peroxidase to quinoneimine