The determination of glucose-6-phosphatase activity in blood serum
β Scribed by J. Kellen; K. Belaj
- Book ID
- 115816398
- Publisher
- Elsevier Science
- Year
- 1961
- Tongue
- English
- Weight
- 160 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0009-8981
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
We present a method to determine glucose 6-phosphate activity. This assay measures the rate of glucose released in the glucose-6-phosphatase reaction. The glucose is oxidized to beta-D-gluconolactone by glucose dehydrogenase in a coupled reaction that uses NAD(P)+. The determination is rapid, reprod
A sensitive and rapid spectrophotometric method for determination of glucose-6-phosphatase activity is described. Glucose formed by the enzyme is oxidized by glucose oxidase to gluconolactone and hydrogen peroxide. The latter, phenol, and 4-aminoantipyrine are converted by peroxidase to quinoneimine