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The control region of the F sex factor DNA transfer cistrons: Restriction mapping and DNA cloning

✍ Scribed by Thompson, Russell ;Achtman, Mark

Publisher: Springer
Year: 1978
Tongue: English
Weight: 880 KB
Volume: 165
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00332530

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✦ Synopsis

A restriction endonuclease map of EcoRI fragment f6 of F sex factor DNA was constructed and aligned with pre-existing physical and genetic maps. Results of genetic complementation tests and analysis of proteins synthesized in minicells from PstI and BglII sub-fragment clones, or from a specific BglII fragment deletion, have allowed mapping of the locations of the origin of DNA transfer and many of the transfer genes known to lie on f6. The proteins detected account for 78% of the coding capacity of fragment f6.

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