𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli

✍ Scribed by Palmer, Margaret L. ;Raker, Mary Alice ;Kennedy, Poindexter J. ;Young, Jon W. ;Barnes, Wayne M. ;Rodriguez, Raymond L. ;Noller, Harry F.


Publisher
Springer
Year
1979
Tongue
English
Weight
731 KB
Volume
172
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

✦ Synopsis


Recombinant plasmids containing the entire 16S RNA gene from the rrn B cistron of E. coli inserted in Col E1 and pBR322 plasmid vectors have been constructed. These plasmids have been mapped using several restriction endonucleases as well as by DNA-RNA hybridization. These maps reveal previously undetected restriction sites in the rrn B cistron and in Col E1 plasmid DNA.