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Promoter mapping and DNA sequencing of the F plasmid transfer genes traM and traJ

✍ Scribed by Thompson, Russell ;Taylor, Linda

Publisher: Springer
Year: 1982
Tongue: English
Weight: 661 KB
Volume: 188
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00330058

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✦ Synopsis

The nucleotide sequence of the DNA encoding the traM, finP and the promoter proximal segment of the traJ gene of the F plasmid has been determined. The predicted amino acid sequence for the traM protein shows that this inner membrane protein contains no signal sequence. The promoters for both the traM and traJ genes have been mapped by in vitro transcription and nuclease S1 protection experiments. No unambiguous location can be assigned to the finP gene but all candidates, if translated, would encode small proteins of between 24 and 52 amino acids.

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