𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The complete chirospectroscopic signature of the peptide 310-helix in aqueous solution

✍ Scribed by Claudio Toniolo; Fernando Formaggio; Sabrina Tognon; Quirinus B. Broxterman; Bernard Kaptein; Rong Huang; Vladimir Setnicka; Timothy A. Keiderling; Iain H. McColl; Lutz Hecht; Laurence D. Barron

Book ID
101719271
Publisher
Wiley (John Wiley & Sons)
Year
2004
Tongue
English
Weight
242 KB
Volume
75
Category
Article
ISSN
0006-3525

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

We synthesized by solution methods a water‐soluble, terminally blocked heptapeptide based on five markedly helicogenic, C^α^‐tetrasubstituted α‐amino acids C^α^‐methyl‐L‐norvalines and two strongly hydrophilic 2‐amino‐3‐[1‐(1,4,7‐triazacyclononane)]‐L‐propanoic acid residues at positions 2 and 5. A Fourier transform infrared absorption and NMR analysis in deuterated chloroform and aqueous solutions of the heptapeptide and two side‐chain protected synthetic precursors confirmed our working hypothesis that all oligomers are folded in the 3~10~‐helical conformation. Based on these findings, we exploited this heptapeptide as a chiral reference compound for detailed electronic CD, vibrational CD, and Raman optical activity characterizations of the 3~10~‐helix in aqueous solution. © 2004 Wiley Periodicals, Inc. Biopolymers, 2004

📜 SIMILAR VOLUMES

Theoretical study of volume changes asso
Theoretical study of volume changes associated with the helix–coil transition of an alanine-rich peptide in aqueous solution
✍ Takashi Imai; Takahiro Takekiyo; Andriy Kovalenko; Fumio Hirata; Minoru Kato; Yo 📂 Article 📅 2005 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 152 KB 👁 1 views

## Abstract The changes in the partial molar volume (PMV) associated with the conformational transition of an alanine‐rich peptide AK16 from the α‐helix structure to various random coil structures are calculated by the three‐dimensional interaction site model (3D‐RISM) theory coupled with the Kirkw

Tat cell-penetrating peptide has the cha
Tat cell-penetrating peptide has the characteristics of a poly(proline) II helix in aqueous solution and in SDS micelles
✍ Paolo Ruzza; Andrea Calderan; Andrea Guiotto; Alessio Osler; Gianfranco Borin 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 76 KB

## Abstract Tat cell‐penetrating peptide (GRKKRRQRRRPPQG) is able to translocate and carry molecules across cell membranes. Using CD spectroscopy the conformation of this synthetic peptide was studied in aqueous and membrane‐mimicking, micellar SDS solutions at different temperatures. The CD spectr

Kinetic studies of the helix–coil transi
Kinetic studies of the helix–coil transition in aqueous solutions of poly(L-lysine)
✍ S. Inoue; T. Sano; Y. Yakabe; H. Ushio; T. Yasunaga 📂 Article 📅 1979 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 544 KB

## Abstract The rate of conformational change of aqueous poly(α‐L‐lysine) solutions was measured using the electric field pulse relaxation method with conductivity detection. The relaxation time as a function of pH exhibits two maxima. One is assigned to a proton transfer reaction and the other to

Conformation of the peptide hormone soma
Conformation of the peptide hormone somatostatin in aqueous solution and organic solvents
✍ Michael Jackson; Henry H. Mantsch 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 368 KB

Fourier transform infrared spectroscopy was applied to the conformational analysis of somatostatin in H,O, 'H,O, dimethyl sulphoxide (DMSO)-'H,O and DMSO. The results suggest that in these solvents the peptide adopts a similar structure. In agreement with circular dichroism (CD) spectroscopic data,