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Tat cell-penetrating peptide has the characteristics of a poly(proline) II helix in aqueous solution and in SDS micelles

✍ Scribed by Paolo Ruzza; Andrea Calderan; Andrea Guiotto; Alessio Osler; Gianfranco Borin


Book ID
105360369
Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
76 KB
Volume
10
Category
Article
ISSN
1075-2617

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✦ Synopsis


Abstract

Tat cell‐penetrating peptide (GRKKRRQRRRPPQG) is able to translocate and carry molecules across cell membranes. Using CD spectroscopy the conformation of this synthetic peptide was studied in aqueous and membrane‐mimicking, micellar SDS solutions at different temperatures. The CD spectrum of the Tat cell‐penetrating peptide in SDS micellar solution was virtually unchanged from that in aqueous solution, and at low temperature it was close to that of a poly(proline) II helix. Copyright © 2004 European Peptide Society and John Wiley & Sons, Ltd.