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The biosynthesis of 13C labeled starch granules as a source of uniformly labeled 13C D-glucose

โœ Scribed by J. R. Buchholz; C. W. Christenson; R. T. Eakin; E. Fowler

Publisher
John Wiley and Sons
Year
1973
Tongue
French
Weight
495 KB
Volume
9
Category
Article
ISSN
0022-2135

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โœฆ Synopsis

Abstract

A potato plant has been grown in a closed chamber with CO~2~ enriched to a level of 31 at. % in ^13^C as the sole source of carbon. In 80 days the plant produced 1,684 g of tubers labeled with 29 at. % ^13^C. of the carbon fixed by the potato plant appromixately 70% was found in the tubers while 30% was found in the tops and roots. Recovered starch granules constituted 10% of the fresh weight of these tubers. A 16% yield of glucose was obtained, based on all of the carbon dioxide used. Distribution of the ^13^C label among the six species of carbon atoms in strach appears to be nearly uniform. The plant and tubers were indistinguishable from those grown on normal isotopic CO~2~ indicating no gross isotope effect at this level of ^13^C enrichement.

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