Teratogenicity induced in cultured rat embryos by the serum of procarbazine treated rats
β Scribed by B.P. Schmid; A. Trippmacher; A. Bianchi
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 473 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0300-483X
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract The growth factor for postimplantation rat embryos was investigated on the basis of the serum speciesβspecificity in supporting embryonic development in culture. We used rabbit serum as a basal medium for the culture of headβfold stage rat embryos, and examined the effects of various fr
## Abstract We attempted to grow explanted 9Β½βday rat embryos in vitro in human serum but were unable to demonstrate normal growth as has been previously described. The embryos were consistently smaller, anaemic, and frequently deformed. These abnormalities were overcome by supplementing the human
An in vivo-in vitro test system with high sensitivity to teratogens has been developed and validated. A single acute intra-peritoneal injection of teratogens (1 8) and non-teratogens (13) was administered to pregnant rats on the 12th day after fertilisation, and uteri were removed after 16 h by lapa
Head fold stage rat embryos were cultured on a medium consisting of adult rat serum for 48 hr. During the final 10 or 20 hr of culture [35S]-methionine was added to the medium. Sera from visceral yolk sac blood vessels, exocoelomic cavity fluid, and amniotic sac fluid were analyzed by SDSpolyacrylam