Temperature Reduction in Cultures of hGM-CSF-expressing CHO Cells: Effect on Productivity and Product Quality
✍ Scribed by Mariela Bollati-Fogolín; Guillermina Forno; Manfred Nimtz; Harald S. Conradt; Marina Etcheverrigaray; Ricardo Kratje
- Book ID
- 109387138
- Publisher
- American Institute of Chemical Engineers
- Year
- 2008
- Tongue
- English
- Weight
- 95 KB
- Volume
- 21
- Category
- Article
- ISSN
- 8756-7938
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## Abstract To investigate the effect of culture temperature on erythropoietin (EPO) production and glycosylation in recombinant Chinese hamster ovary (CHO) cells, we cultivated CHO cells using a perfusion bioreactor. Cells were cultivated at 37°C until viable cell concentration reached 1 × 10^7^ c
As we pursue the means to improve yields to meet growing therapy demands, it is important to examine the impact of process control on glycosylation patterns to ensure product efficacy and consistency. In this study, we describe a dynamic on-line fed-batch strategy based on low glutamine/glucose conc