Use of a small molecule cell cycle inhibitor to control cell growth and improve specific productivity and product quality of recombinant proteins in CHO cell cultures
โ Scribed by Du, Zhimei; Treiber, David; McCarter, John D.; Fomina-Yadlin, Dina; Saleem, Ramsey A.; McCoy, Rebecca E.; Zhang, Yuling; Tharmalingam, Tharmala; Leith, Matthew; Follstad, Brian D.; Dell, Brad; Grisim, Brent; Zupke, Craig; Heath, Carole; Morris, Arvia E.; Reddy, Pranhitha
- Book ID
- 127231119
- Publisher
- John Wiley and Sons
- Year
- 2014
- Tongue
- English
- Weight
- 764 KB
- Volume
- 112
- Category
- Article
- ISSN
- 0006-3592
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The use of a temperature switch to control the growth and productivity of temperature-sensitive (ts) mutants was investigated to extend the productive life span of recombinant Chinese hamster ovary (CHO) cells in batch culture. Bromodeoxyuridine was used at 39ยฐC to select mutagenized CHO-K1 cells, w
## Abstract Plant cell culture production of recombinant products offers a number of advantages over traditional eukaryotic expression systems, particularly if the product can be targeted to and purified from the cell culture broth. However, one of the main obstacles is product degradation by prote