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Use of cell cycle analysis to characterise growth and interferon-γ production in perfusion culture of CHO cells

✍ Scribed by Vichai Leelavatcharamas; A. Nichola Emery; M. Al-Rubeai

Book ID
110257996
Publisher
Springer
Year
1999
Tongue
English
Weight
212 KB
Volume
30
Category
Article
ISSN
0920-9069

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## Abstract To investigate the effect of culture temperature on erythropoietin (EPO) production and glycosylation in recombinant Chinese hamster ovary (CHO) cells, we cultivated CHO cells using a perfusion bioreactor. Cells were cultivated at 37°C until viable cell concentration reached 1 × 10^7^ c