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Temperature Effects on Product-Quality-Related Enzymes in Batch CHO Cell Cultures Producing Recombinant tPA

โœ Scribed by Kevin J. R. Clark; Frank W. R. Chaplin; Sarah W. Harcum


Book ID
109387198
Publisher
American Institute of Chemical Engineers
Year
2004
Tongue
English
Weight
141 KB
Volume
20
Category
Article
ISSN
8756-7938

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## Abstract To investigate the effect of culture temperature on erythropoietin (EPO) production and glycosylation in recombinant Chinese hamster ovary (CHO) cells, we cultivated CHO cells using a perfusion bioreactor. Cells were cultivated at 37ยฐC until viable cell concentration reached 1โ€‰ร—โ€‰10^7^ c