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Targeted mutagenesis in zebrafish using customized zinc-finger nucleases

✍ Scribed by Foley, Jonathan E; Maeder, Morgan L; Pearlberg, Joseph; Joung, J Keith; Peterson, Randall T; Yeh, Jing-Ruey J

Book ID: 109946713
Publisher: Nature Publishing Group
Year: 2009
Tongue: English
Weight: 765 KB
Volume: 4
Category: Article
ISSN: 1750-2799
DOI: 10.1038/nprot.2009.209

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The ability to achieve site-specific manipulation of the mammalian genome has widespread implications for basic and applied research. Gene targeting is a process in which a DNA molecule introduced into a cell replaces the corresponding chromosomal segment by homologous recombination, and thus presen