Tangential flow filtration for virus purification
β Scribed by David L. Grzenia; Jonathan O. Carlson; S. Ranil Wickramasinghe
- Book ID
- 104054839
- Publisher
- Elsevier Science
- Year
- 2008
- Tongue
- English
- Weight
- 794 KB
- Volume
- 321
- Category
- Article
- ISSN
- 0376-7388
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π SIMILAR VOLUMES
A simple, scalable method for purification of plasmid DNA is described. The method includes modification of the classical alkaline-lysis-based plasmid extraction method by extending the solubilization step from less than 30 min to 24 h. The extraction is followed by the novel use of tangential flow
## Abstract A recent study by __Palmer, Sun, and Harris (Biotechnol. Prog__., 25:189β199, 2009) demonstrated that tangential flow filtration (TFF) can be used to produce HPLCβgrade bovine and human hemoglobin (Hb). In this current study, we assessed the quality of bovine Hb (bHb) purified by introd