Purification of PEGylated Nanoparticles Using Tangential Flow Filtration (TFF)
β Scribed by Dalwadi, Gautam; Sunderland, Vivian Bruce
- Book ID
- 118065375
- Publisher
- Informa plc
- Year
- 2007
- Tongue
- English
- Weight
- 316 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0363-9045
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π SIMILAR VOLUMES
A simple, scalable method for purification of plasmid DNA is described. The method includes modification of the classical alkaline-lysis-based plasmid extraction method by extending the solubilization step from less than 30 min to 24 h. The extraction is followed by the novel use of tangential flow
## Abstract A recent study by __Palmer, Sun, and Harris (Biotechnol. Prog__., 25:189β199, 2009) demonstrated that tangential flow filtration (TFF) can be used to produce HPLCβgrade bovine and human hemoglobin (Hb). In this current study, we assessed the quality of bovine Hb (bHb) purified by introd