A simple, scalable method for purification of plasmid DNA is described. The method includes modification of the classical alkaline-lysis-based plasmid extraction method by extending the solubilization step from less than 30 min to 24 h. The extraction is followed by the novel use of tangential flow
Purification of infectious adenovirus in two hours by ultracentrifugation and tangential flow filtration
β Scribed by Hideyo Ugai; Takahito Yamasaki; Megumi Hirose; Kumiko Inabe; Yukari Kujime; Miho Terashima; Bingbing Liu; Hong Tang; Mujun Zhao; Takehide Murata; Makoto Kimura; Jianzhi Pan; Yuichi Obata; Hirofumi Hamada; Kazunari K. Yokoyama
- Book ID
- 116290420
- Publisher
- Elsevier Science
- Year
- 2005
- Tongue
- English
- Weight
- 513 KB
- Volume
- 331
- Category
- Article
- ISSN
- 0006-291X
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
There is an increasing interest in the development of scaleable and reproducible plasmid DNA purification protocols for vaccine and gene therapy. The use of an integrated unit operation, comprising tangential flow microfiltration coupled with the adsorption of contaminants onto nitrocellulose membra
The circulation of microorganisms in tangential flow filtration systems induces perturbations and then the damage of brittle cells. This work is focused on the shearing of two marine microalgae species (Skeletonema costatum and Haslea ostrearia), both largely culti- vated in western France (Re Β΄gion
## Abstract Highβperformance tangential flow filtration (HPTFF) is shown to successfully enable concentration, purification and formulation in a single unit operation. This is illustrated with feedstreams comprising recombinant proteins expressed in __Escherichia coli__ (__E. coli__). Using positiv