𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Purification of infectious adenovirus in two hours by ultracentrifugation and tangential flow filtration

✍ Scribed by Hideyo Ugai; Takahito Yamasaki; Megumi Hirose; Kumiko Inabe; Yukari Kujime; Miho Terashima; Bingbing Liu; Hong Tang; Mujun Zhao; Takehide Murata; Makoto Kimura; Jianzhi Pan; Yuichi Obata; Hirofumi Hamada; Kazunari K. Yokoyama


Book ID
116290420
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
513 KB
Volume
331
Category
Article
ISSN
0006-291X

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


Purification of plasmid DNA by tangentia
✍ David W. Kahn; Michelle D. Butler; Darien L. Cohen; Margaret Gordon; Jeanne W. K πŸ“‚ Article πŸ“… 2000 πŸ› John Wiley and Sons 🌐 English βš– 165 KB πŸ‘ 1 views

A simple, scalable method for purification of plasmid DNA is described. The method includes modification of the classical alkaline-lysis-based plasmid extraction method by extending the solubilization step from less than 30 min to 24 h. The extraction is followed by the novel use of tangential flow

Purification of plasmid DNA by an integr
✍ D. Kendall; G. J. Lye; M. S. Levy πŸ“‚ Article πŸ“… 2002 πŸ› John Wiley and Sons 🌐 English βš– 176 KB

There is an increasing interest in the development of scaleable and reproducible plasmid DNA purification protocols for vaccine and gene therapy. The use of an integrated unit operation, comprising tangential flow microfiltration coupled with the adsorption of contaminants onto nitrocellulose membra

Effects of shear on two microalgae speci
✍ L. Vandanjon; N. Rossignol; P. Jaouen; J. M. Robert; F. QuΓ©mΓ©neur πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 441 KB πŸ‘ 1 views

The circulation of microorganisms in tangential flow filtration systems induces perturbations and then the damage of brittle cells. This work is focused on the shearing of two marine microalgae species (Skeletonema costatum and Haslea ostrearia), both largely culti- vated in western France (Re Β΄gion

Application of high-performance tangenti
✍ BΓ©nΓ©dicte Lebreton; Arick Brown; Robert van Reis πŸ“‚ Article πŸ“… 2008 πŸ› John Wiley and Sons 🌐 English βš– 247 KB

## Abstract High‐performance tangential flow filtration (HPTFF) is shown to successfully enable concentration, purification and formulation in a single unit operation. This is illustrated with feedstreams comprising recombinant proteins expressed in __Escherichia coli__ (__E. coli__). Using positiv