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Purification of Densonucleosis Virus by Tangential Flow Ultrafiltration

✍ Scribed by David L. Grzenia; Jonathan O. Carlson; Peter Czermak; Binbing Han; Rachel K. Specht; S. Ranil Wickramasinghe

Book ID
109387423
Publisher
American Institute of Chemical Engineers
Year
2008
Tongue
English
Weight
343 KB
Volume
22
Category
Article
ISSN
8756-7938

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Purification of plasmid DNA by tangentia
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A simple, scalable method for purification of plasmid DNA is described. The method includes modification of the classical alkaline-lysis-based plasmid extraction method by extending the solubilization step from less than 30 min to 24 h. The extraction is followed by the novel use of tangential flow