Taking the Pulse of Ribosome Assembly In Vivo
โ Scribed by Walter E. Hill
- Book ID
- 116664475
- Publisher
- Elsevier Science
- Year
- 2010
- Tongue
- English
- Weight
- 74 KB
- Volume
- 403
- Category
- Article
- ISSN
- 0022-2836
No coin nor oath required. For personal study only.
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## Abstract GTPases are a universally conserved class of regulatory proteins involved in such diverse cellular functions as signal transduction, translation, cytoskeleton formation, and intracellular transport. GTPases are also required for ribosome assembly in eukaryotes and bacteria, where they p
Functionally active 30s ribosomes can be reconstituted i n vitro from 16s RNA and a mixture of 305 ribosomal proteins under certain defined conditions. Our previous studies on the specificity and kinetics of reconstitution are summarized and discussed. The reconstitution reaction is first-order with