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T Antigen Origin-Binding Domain of Simian Virus 40: Determinants of Specific DNA Binding †

✍ Scribed by Bradshaw, Elizabeth M.; Sanford, David G.; Luo, Xuelian; Sudmeier, James L.; Gurard-Levin, Zachary A.; Bullock, Peter A.; Bachovchin, William W.

Book ID
126132849
Publisher
American Chemical Society
Year
2004
Tongue
English
Weight
144 KB
Volume
43
Category
Article
ISSN
0006-2960

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Simian Virus 40 T Antigen Binds to DNA
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Structure-based design of a disulfide-li
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With the aim of forming the ‘lock-washer’ conformation of the origin-binding domain of SV40 large T antigen in solution, using structure-based analysis an intermolecular disulfide bridge was engineered into the origin-binding domain to generate higher order oligomers in solution. The 1.7 Å resolutio