✦ LIBER ✦
Structure-based design of a disulfide-linked oligomeric form of the simian virus 40 (SV40) large T antigen DNA-binding domain
✍ Scribed by Meinke, Gretchen ;Phelan, Paul ;Fradet-Turcotte, Amélie ;Archambault, Jacques ;Bullock, Peter A.
- Publisher
- International Union of Crystallography
- Year
- 2011
- Tongue
- English
- Weight
- 789 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0907-4449
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✦ Synopsis
With the aim of forming the ‘lock-washer’ conformation of the origin-binding domain of SV40 large T antigen in solution, using structure-based analysis an intermolecular disulfide bridge was engineered into the origin-binding domain to generate higher order oligomers in solution. The 1.7 Å resolution structure shows that the mutant forms a spiral in the crystal and has the de novo disulfide bond at the protein interface, although structural rearrangements at the interface are observed relative to the wild type.