Purification of ribonuclease T1
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C.Nick Pace; Gerald R. Grimsley; Ben J. Barnett
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Article
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1987
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Elsevier Science
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English
โ 441 KB
An improved method for purifying ribonuclease T1 from Aspergillus oryzae is described. The method uses gradient elution from DEAE-cellulose and sulfopropyl-Sephadex columns followed by gel filtration on Sephadex G-50 to give almost 100 mg (50% yield) of ribonuclease T1 from 100 g of starting materia