Improving purification of recombinant ribonuclease T1
โ Scribed by Olfert Landt; Maria Zirpel-Giesebrecht; Andreas Milde; Ulrich Hahn
- Book ID
- 119131789
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 290 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0168-1656
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๐ SIMILAR VOLUMES
An improved method for purifying ribonuclease T1 from Aspergillus oryzae is described. The method uses gradient elution from DEAE-cellulose and sulfopropyl-Sephadex columns followed by gel filtration on Sephadex G-50 to give almost 100 mg (50% yield) of ribonuclease T1 from 100 g of starting materia
Purification of ribonuclease T, has been achieved using a porous glass-2',(3')-GMP affinity adsorbent. This adsorbent cshibits specific affinity for ribonuclease T, and is almost devoid of non-specific ion-eschange properties. \_.~.\_.. .\_.