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A Purification Method for Labile Variants of Ribonuclease T1

โœ Scribed by L.M. Mayr; F.X. Schmid

Book ID
115644730
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
606 KB
Volume
4
Category
Article
ISSN
1046-5928

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We describe a straightforward and simple method for obtaining pure and active preparations of type 1 ribosome inactivating proteins (RIPs). The very high isoelectric point values, characteristic of these proteins, allow this purification in a single chromatographic step.