๐”– Bobbio Scriptorium
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Purification of ribonuclease T1

โœ Scribed by C.Nick Pace; Gerald R. Grimsley; Ben J. Barnett

Book ID
102628689
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
441 KB
Volume
167
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

An improved method for purifying ribonuclease T1 from Aspergillus oryzae is described. The method uses gradient elution from DEAE-cellulose and sulfopropyl-Sephadex columns followed by gel filtration on Sephadex G-50 to give almost 100 mg (50% yield) of ribonuclease T1 from 100 g of starting material in less than 5 days.

๐Ÿ“œ SIMILAR VOLUMES

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