𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Synthesis of [γ-32P]thiamine triphosphate

✍ Scribed by Ch. Grandfils; L. Bettendorff; C. de Rycker; E. Schoffeniels

Book ID
102630223
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
423 KB
Volume
169
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

We developed a novel chemical synthesis of thiamine triphosphate which allows us to incorporate 32P in the gamma position. The reaction is based on the condensation of [32P]orthophosphoric acid and thiamine diphosphate in the presence of ethyl chloroformate. After purification by two ion-exchange purification steps, the thiamine derivative has a specific radioactivity of 10 Ci/mmol. The average final yield synthesis is about 10%.

📜 SIMILAR VOLUMES

A simple method for synthesizing [γ-32P]
A simple method for synthesizing [γ-32P]nucleoside triphosphates using [32P]acetylphosphate and acetate kinase
✍ Pál I. Bauer; Gyula Várady 📂 Article 📅 1978 🏛 Elsevier Science 🌐 English ⚖ 256 KB

A simple, rapid, and inexpensive method is described for the synthesis of gamma-32P-labeled ribo- or deoxyribonucleoside triphosphates. The procedure involves chemical synthesis of [32P]acetylphosphate and subsequent phosphorylation of nucleoside diphosphates using acetate kinase (EC 2.7.2.1) and a