We developed a novel chemical synthesis of thiamine triphosphate which allows us to incorporate 32P in the gamma position. The reaction is based on the condensation of [32P]orthophosphoric acid and thiamine diphosphate in the presence of ethyl chloroformate. After purification by two ion-exchange pu
✦ LIBER ✦
A chemical synthesis of adenosine 5′-[γ-32P]triphosphate
✍ Scribed by Sidney M. Hecht; John W. Kozarich
- Book ID
- 115745132
- Publisher
- Elsevier Science
- Year
- 1973
- Weight
- 130 KB
- Volume
- 331
- Category
- Article
- ISSN
- 0005-2787
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