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Synthesis of the biologically active β-subunit of human nerve growth factor in Escherichia coli

✍ Scribed by Negro, Alessandro; Martini, Irene; Bigon, Emilio; Cazzola, Flavia; Minozzi, Cristina; Skaper, Stephen D.; Callegaro, Lanfranco

Book ID
123271971
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
546 KB
Volume
110
Category
Article
ISSN
0378-1119

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The complete mouse prepro-nerve growth factor (NGF) DNA was fused to the carboxyl terminus of the 6-galactosidase (lac-z) gene of Escherichia coli. Similarly, a genomic fragment encoding the human NGF comprising codons 11 to 106 (from a total of 118) was fused to the fifth codon of the amino terminu