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Expression of human β-subunit nerve growth factor (hNGFB) in yeastPichia pastorisandE. coli

✍ Scribed by Qiao Fan; Hongdi Liu; Tong Sun; Rongqiao He

Book ID
105642409
Publisher
Springer
Year
1999
Tongue
English
Weight
475 KB
Volume
44
Category
Article
ISSN
1001-6538

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The complete mouse prepro-nerve growth factor (NGF) DNA was fused to the carboxyl terminus of the 6-galactosidase (lac-z) gene of Escherichia coli. Similarly, a genomic fragment encoding the human NGF comprising codons 11 to 106 (from a total of 118) was fused to the fifth codon of the amino terminu