Synthesis of chimeric mouse nerve growth factor precursor and human β-nerve growth factor in Escherichia coli: Immunological properties

✍ Scribed by E. Dicou; R. Houlgatte; J. Lee; B. von Wilcken-Bergmann

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 670 KB
• Volume: 22
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.490220103

✦ Synopsis

The complete mouse prepro-nerve growth factor (NGF) DNA was fused to the carboxyl terminus of the 6-galactosidase (lac-z) gene of Escherichia coli. Similarly, a genomic fragment encoding the human NGF comprising codons 11 to 106 (from a total of 118) was fused to the fifth codon of the amino terminus of Pgalactosidase. Both bacterial vectors produce high amounts of the chimeric proteins. After cell lysis most of the chimeric mouse preproNGF protein is insoluble and appears in the pellet, whereas the majority of the chimeric human P-NGF remains in the supernatant.

Purification of the fusion proteins from the soluble fraction was achieved by affinity chromatography to p-aminophenyl P-D-thio-galactoside Sepharose. Yields of the purified chimeric proteins were increased threefold to fourfold by the addition of protease inhibitors in the lysis and chromatography buffers. Their antigenic similarity to the preproNGF and mouse 0-NGF was examined by their interaction to sera raised against synthetic peptides which reproduce sequences of the precursor protein and to sera directed against native and denatured mouse 0-NGF using enzymelinked immunoabsorbent assay (ELISA) techniques. Antibodies to the peptide N2 (-163 to -139) interacted with high affinity with the chimeric mouse preproNGF protein. Antisera to native and denatured mouse P-NGF interacted with both chimeric proteins but with a variable degree of affinity. These results provide direct evidence that certain antisera to mouse 0-NGF can cross-react with the human P-NGF molecule.