𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Rat β-nerve growth factor sequence and site of synthesis in the adult hippocampus

✍ Scribed by Dr. S. R. Whittemore; P. L. Friedman; D. Larhammar; H. Persson; M. Gonzalez-Carvajal; V. R. Holets

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
899 KB
Volume
20
Category
Article
ISSN
0360-4012

No coin nor oath required. For personal study only.

✦ Synopsis

A rat p-nerve growth factor (NGF) genomic sequence encoding the entire 3' exon of preproNGF was cloned, and its nucleotide sequence was determined. Rat NGF shows very high homology with other known NGFs in both the prepropeptide and the 3' untranslated regions. The presumptive signal sequence, the cysteine residues important for tertiary structure, possible glycosylation sites, and dibasic amino acids required for proteolytic cleavage to mature NGF are conserved across species. Comparison of the hydrophobicity plots and amino acid sequences revealed an evolutionary divergent domain on the external surface of NGF, which may account for the poor immunologic crossreactivities of the various NGFs. In situ hybridization with a ratspecific oligodeoxynucleotide indicated high levels of NGF mRNA synthesis in both hippocampal granule and pyramidal cell layers. These results are consistent with one role for NGF in the CNS as a neuronally released, retrogradely transported neurotrophic factor for basal forebrain cholinergic neurons.

📜 SIMILAR VOLUMES

Nerve growth factor protein level increa
Nerve growth factor protein level increases in the adult rat hippocampus aftera specific cholinergic lesion
✍ L. Lärkfors; I. Strömberg; T. Ebendal; L. Olson 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 817 KB

Nerve growth factor (NGF) is the best-characterized neurotrophic substance and has recently been shown to influence cholinergic neurons in the basal forebrain. Hippocampus and neocortex, the primary targets for these central neurons, have further been found to contain high levels of NGF. Using enzym

Intraspinal and behavioral consequences
Intraspinal and behavioral consequences of nerve growth factor-induced nociceptive sprouting and nerve growth factor-induced hyperalgesia compared in adult rats
✍ Pertens, Evi; Urschel-Gysbers, Bethany A.; Holmes, Michael; Pal, Raveen; Foerste 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 454 KB 👁 2 views

Intraspinal and behavioral events were studied in adult rats with nociceptive nerves that were undergoing collateral sprouting into adjacent denervated skin. This sprouting, which is driven by endogenous nerve growth factor (NGF), did not cause hyperalgesia. For comparison, we studied an exogenous N

Synthesis of chimeric mouse nerve growth
Synthesis of chimeric mouse nerve growth factor precursor and human β-nerve growth factor in Escherichia coli: Immunological properties
✍ E. Dicou; R. Houlgatte; J. Lee; B. von Wilcken-Bergmann 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 670 KB

The complete mouse prepro-nerve growth factor (NGF) DNA was fused to the carboxyl terminus of the 6-galactosidase (lac-z) gene of Escherichia coli. Similarly, a genomic fragment encoding the human NGF comprising codons 11 to 106 (from a total of 118) was fused to the fifth codon of the amino terminu

Neuroprotective effects of estradiol in
Neuroprotective effects of estradiol in the adult rat hippocampus: Interaction with insulin-like growth factor-I signalling
✍ Iñigo Azcoitia; Amanda Sierra; Luis M. Garcia-Segura 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 251 KB 👁 1 views

We have previously shown that 17-␤-estradiol protects neurons in the dentate gyrus from kainic acidinduced death in vivo. To analyse whether this effect is mediated through estrogen receptors and through cross-talk between steroid and insulin-like growth factor (IGF) systems, we have concomitantly a

Serotonin regulation of nerve growth fac
Serotonin regulation of nerve growth factor synthesis in neonatal and adult astrocytes: Comparison to the β-adrenergic agonist isoproterenol
✍ Mojca Krzan; Vivian W. Wu; Joan P. Schwartz 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 147 KB

Although serotonin regulates synthesis of the neurotrophic factor S-100 beta by astrocytes, its ability to affect nerve growth factor (NGF) synthesis has never been examined. We report here that there is a correlation between the effect of serotonin on cyclic adenosine monophosphate (cAMP) content a

Microsphere embolism-induced elevation o
Microsphere embolism-induced elevation of nerve growth factor level and appearance of nerve growth factor immunoreactivity in activated T-lymphocytes in the rat brain
✍ Hideyuki Mizuma; Kaori Takagi; Keiko Miyake; Norio Takagi; Kumi Ishida; Satoshi 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 236 KB 👁 2 views

Changes in nerve growth factor (NGF) level and type of cells producing NGF were investigated in the rat brain after sustained cerebral embolism. The NGF level was determined by a two-site enzyme immunoassay specific for NGF. The cerebral cortex, striatum, and hippocampus of the embolized hemisphere