Synthesis of rat muscle carbonic anhydrase III in a cell-free translation system
β Scribed by Alan Shiels; Ian Phillips; Stephen Jeffrey; Elizabeth Shephard; Nicholas Carter
- Book ID
- 115914059
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 729 KB
- Volume
- 148
- Category
- Article
- ISSN
- 0014-5793
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A cell-free translation system producing mature green fluorescent protein (GFP) can be a useful tool for studying the mechanism and kinetics of GFP chromophore formation, as well as for fast protein engineering. We report here that the mature GFP can be formed in the cellfree translation system from