Two modifications are introduced to convert the Escherichia coli cell-free extract ("S30") into a high efficiency system for coupled transcription/translation of exogenously added genes. (a) The ribosome fraction collected from the S30 by ultracentrifugation is used. It contains all the proteins nec
Synthesis of colicin E1 in a cell-free coupled transcription-translation system
โ Scribed by V. V. Emel'yanov; V. N. Kalinin; B. M. Girdo; T. I. Tikhonenko
- Publisher
- Springer US
- Year
- 1976
- Tongue
- English
- Weight
- 206 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0007-4888
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A cell-free translation system producing mature green fluorescent protein (GFP) can be a useful tool for studying the mechanism and kinetics of GFP chromophore formation, as well as for fast protein engineering. We report here that the mature GFP can be formed in the cellfree translation system from
## Abstract Herewith we report the encapsulation of functional protein synthesis machinery in a silica solโgel matrix. When the solโgel reaction using alkoxysilane monomers was carried out in the presence of __Escherichia coli__ cell extract, macromolecular protein synthesis machinery in the cell e
Highly purified feather keratin mRNA, prepared by dissociation of mRNP particles in Na dodecyl sulphate, was translated in a wheat embryo cell-free system with similar efficiency to rabbit globin mRNA and RNA purified from cucumber mosaic virus. The only detectable products of translation of the ker