๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Synthesis of colicin E1 in a cell-free coupled transcription-translation system

โœ Scribed by V. V. Emel'yanov; V. N. Kalinin; B. M. Girdo; T. I. Tikhonenko


Publisher
Springer US
Year
1976
Tongue
English
Weight
206 KB
Volume
82
Category
Article
ISSN
0007-4888

No coin nor oath required. For personal study only.


๐Ÿ“œ SIMILAR VOLUMES


High efficiency cell-free synthesis of p
โœ Wieslaw Kudlicki; Gisela Kramer; Boyd Hardesty ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 734 KB

Two modifications are introduced to convert the Escherichia coli cell-free extract ("S30") into a high efficiency system for coupled transcription/translation of exogenously added genes. (a) The ribosome fraction collected from the S30 by ultracentrifugation is used. It contains all the proteins nec

Synthesis and maturation of green fluore
โœ Vyacheslav A. Kolb; Eugeny V. Makeyev; William W. Ward; Alexander S. Spirin ๐Ÿ“‚ Article ๐Ÿ“… 1996 ๐Ÿ› Springer Netherlands ๐ŸŒ English โš– 375 KB

A cell-free translation system producing mature green fluorescent protein (GFP) can be a useful tool for studying the mechanism and kinetics of GFP chromophore formation, as well as for fast protein engineering. We report here that the mature GFP can be formed in the cellfree translation system from

Cell-free synthesis of functional protei
โœ Kyeong-Ohn Kim; Seong Yoon Lim; Geun-Hee Hahn; Sahng Ha Lee; Chan Beum Park; Don ๐Ÿ“‚ Article ๐Ÿ“… 2009 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 254 KB

## Abstract Herewith we report the encapsulation of functional protein synthesis machinery in a silica solโ€“gel matrix. When the solโ€“gel reaction using alkoxysilane monomers was carried out in the presence of __Escherichia coli__ cell extract, macromolecular protein synthesis machinery in the cell e

Translation of pure feather keratin mRNA
โœ David J. Kemp; Mark W. Schwinghamer; George E. Rogers ๐Ÿ“‚ Article ๐Ÿ“… 1974 ๐Ÿ› Springer ๐ŸŒ English โš– 291 KB

Highly purified feather keratin mRNA, prepared by dissociation of mRNP particles in Na dodecyl sulphate, was translated in a wheat embryo cell-free system with similar efficiency to rabbit globin mRNA and RNA purified from cucumber mosaic virus. The only detectable products of translation of the ker