Quantification of carbonic anhydrase III and myoglobin in different fiber types of human psoas muscle

✍ Scribed by A. Zheng; P. Rahkila; J. Vuori; S. Rasi; T. Takala; H. K. Väänänen

• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 508 KB
• Volume: 97
• Category: Article
• ISSN: 1432-119X
• DOI: 10.1007/bf00271284

✦ Synopsis

Carbonic anhydrase (CA III) and myoglobin contents from isolated human muscle fibers were quantified using a sensitive time-resolved fluoroimmunoassay. Human psoas muscle specimens were freeze-dried, and single fibers were dissected out and classified into type I, IIA and IIB by myosin ATPase staining. Fiber typing was further confirmed by SDS-PAGE. CA III and myoglobin were found in all fiber types. Type I fibers contained higher concentrations of CA III and myoglobin than type IIA and IIB fibers. The relative concentrations of CA III in type IIA and IIB fibers were respectively 24% and 10% of that in type I fibers. The relative concentrations of myoglobin in type IIA and IIB fibers were 60% and 28% of that in type I fibers. Anti-CA III immunoblotting results from fiber-specific pooled samples agreed well with quantitative measurements. The results indicate that CA III is a more specific marker than myoglobin for type I fibers.